CHANNEL CONTINUOUS MICRO-REACTORS FOR THE PRODUCTION OF BIOPHARMACEUTICALS IN OVARY CELLS OF CHINESE HAMSTER

• Main Authors: LUCÍA DANIELA GARZA GARCÍA, SANDRA OZUNA CHACON, MARIO MOISES ALVAREZ
• Format: Patent
• Language: English; Spanish
• Published: 21.04.2017
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE; CHEMISTRY; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PERFORMING OPERATIONS; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL; SPIRITS; TRANSPORTING; VINEGAR; WINE

The present invention refers to a group of micro-devices that a) have micro-channels with corners ranging from 70° to 110°, particularly but not exclusively those with zig-zag micro-channels; b) have a texture in the surface of the channel that promotes the cellular adherence and the retention or proliferation of the recombinant cells of the mammal, particularly but not exclusively ovary cells of Chinese hamster; c) are fed with a culture medium in a continuous manner by some type of pumping device. This plurality of micro-devices may be used in screening applications for culturing recombinant cells of mammals, particularly but not exclusively CHO cells, or for the production of biopharmaceuticals or other recombinant proteins. La presente invención se refiere a un conjunto de micro-dispositivos que comprende micro-canales con tramos rectos que se unen en esquinas de 70 a 110°, preferentemente de 90°; donde los micro-canales tienen superficies de canal y donde dichas superficies de canal poseen una textura que propicia la adherencia celular y la retención o proliferación de células recombinantes de mamífero, particularmente pero no exclusivamente, células de ovario de hámster chino; un dispositivo de bombeo para alimentar un medio de cultivo en forma continua, caracterizado porque la textura de las superficies del canal consiste de cavidades semiesféricas.