METHOD FOR THE DETECTION OF COLLETOTRICHUM CAPSICI PHYTOPATHOGEN FUNGI USING THE POLYMERASE CHAIN REACTION (PCR)

• Main Authors: INOCENCIO HIGUERA CIAPARA, DAISY DE LA CARIDAD PÉREZ BRITO, CLAUDIA GUADALUPE TORRES CALZADA, ANDRÉS FELIPE DE JESUS QUIJANO RAMAYO, RAÚL TAPIA TUSSELL
• Format: Patent
• Language: English; Spanish
• Published: 21.10.2016
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

Described are initiators, a detection kit and method for detecting Colletotrichum capsici, which comprises the following steps: (a) obtaining a biological sample from the desired plant; (b) isolating the DNA of said biological sample; (c) subjecting the DNA to a polymerase chain reaction (PCR) using as initiator a sense sequence (SEQ ID NO. 1): 5'-GTA GCC GTC CCC TAA AAA GG-3' and an antisense sequence ((SEQ ID NO. 2): 5'-CCC AAT GCG AGA CGA AAT G-3'; and (d) detecting the Colletotrichum capsici by visualizing the amplified product of the polymerase chain reaction. La presente invención se refiere a iniciadores, Kit de detección y método para la detección de Colletotrichum capsici que comprende las etapas de: (a) obtener una muestra biológica de la planta de interés; (b) aislar ADN de dicha muestra biológica; (c) someter el ADN a una reacción en cadena de la polimerasa (PCR) utilizando como iniciadores una secuencia sentido (SEQ ID NO. 1): 5'-GTA GCC GTC CCC TAA AAA GG-3' y una secuencia antisentido (SEQ ID NO. 2): 5'-CCC AAT GCG AGA CGA AAT G-3'; y (d) detectar el Colletotrichum capsici por visualización del producto de amplificación, de la reacción de cadena de polimerasa.