MUTATED SULFOTRANSFERASES AND USES THEREOF

The invention relates to non-naturally occurring mutated arylsulfotransferases comprising (i) an amino acid substitution in at least one amino acid position selected among positions 6, 7, 8, 9, 11, 17, 20, 33, 62, 97, 138, 195, 236, 239, 244, 263, and combinations thereof, wherein the position is re...

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Bibliographic Details
Main Authors DEPLACE Aymeric, MONZA Emanuele, PANIGADA Davide, STEINMETZ Anke
Format Patent
LanguageEnglish
Spanish
Published 28.06.2024
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The invention relates to non-naturally occurring mutated arylsulfotransferases comprising (i) an amino acid substitution in at least one amino acid position selected among positions 6, 7, 8, 9, 11, 17, 20, 33, 62, 97, 138, 195, 236, 239, 244, 263, and combinations thereof, wherein the position is relative to the amino acids sequence of rat arylsulfotransferase IV SEQ ID NO: 1 and (ii) an amino acid sequence having at least 60% sequence identity with amino acids sequence SEQ ID NO: 1. The mutated arylsulfotransferase may have a sulfotransferase activity for converting adenosine 3',5'-bisphosphate (PAP) into 3'-phosphoadenosine-5'-phosphosulfate (PAPS) enhanced compared to the wild-type enzyme. La invención se refiere a arilsulfotransferasas mutadas de origen no natural que comprenden (i) una sustitución de aminoácidos en al menos una posición de aminoácidos seleccionada entre las posiciones 6, 7, 8, 9, 11, 17, 20, 33, 62, 97, 138, 195, 236, 239, 244, 263, y combinaciones de las mismas, en donde la posición es relativa a la secuencia de aminoácidos de la arilsulfotransferasa IV de rata de la SEQ ID NO: 1 y (ii) una secuencia de aminoácidos que tiene al menos un 60 % de identidad de secuencia con la secuencia de aminoácidos SEQ ID NO: 1. La arilsulfotransferasa mutada puede tener una actividad de sulfotransferasa para convertir adenosina 3',5'-bifosfato (PAP) en 3'-fosfoadenosina-5'-fosfosulfato (PAPS) mejorada en comparación con la enzima de tipo silvestre.
Bibliography:Application Number: MX20240003964