METHODS FOR MAKING HIGH INTENSITY SWEETENERS

• Main Authors: ZIELER Helge, FABER Nathan, PATRON Andrew P, NORIEGA Chris Edano, STEGE Justin, COLQUITT Justin, MANAM Rama R
• Format: Patent
• Language: English; Spanish
• Published: 24.04.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; FOODS OR FOODSTUFFS; FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J; HUMAN NECESSITIES; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES; VINEGAR; WINE

Provided herein include methods of making mogroside compounds, e.g., Compound 1, compositions (for example host cells) for making the mogroside compounds, and the mogroside compounds made by the methods disclosed herein, and compositions (for example, cell lysates) and recombinant cells comprising the mogroside compounds (e.g., Compound 1). Also provided herein are novel cucurbitadienol synthases and the use thereof. En la presente se proporcionan la inclusión de métodos para producir compuestos mogrósido, por ejemplo, el compuesto 1, composiciones (por ejemplo, células hospedadoras) para producir los compuestos mogrósido, y los compuestos mogrósido elaborados por los métodos descritos en la presente, y composiciones (por ejemplo, lisados celulares) y células recombinantes que comprenden los compuestos mogrósido (por ejemplo, el compuesto 1). También se proporcionan en la presente cucurbitadienol sintasa novedosas y el uso de las mismas.