SPECIFIC PRIMERS AND STANDARDIZED METHOD FOR DETECTING MYCOPLASMA GENITALIUM IN CERVICOVAGINAL AND URETHRAL EXUDATE SAMPLES

• Main Authors: AGUILERA ARREOLA Ma. Guadalupe, GÓMEZ LUNAR Zulema, HERNÁNDEZ CORTEZ Cecilia, CONTRERAS RODRÍGUEZ Araceli, MORALES GARCÍA María Del Rosario Jovita, RUIZ CASTILLO Enrico Alejandro
• Format: Patent
• Language: English; Spanish
• Published: 14.06.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

The present invention aims to provide a set of specific primers, the method, and its integration into a kit for the unique identification of Mycoplasma genitalium by the PCR technique using the particular primers SEQ and SEQ 2, and including the use of the appropriate concentrations of reagents for the unique amplification, as well as the amplification of at least one negative control and one positive control. Also, it provides a rapid and susceptible method for use with any PCR equipment for molecular biology laboratories with basic infrastructure, using the appropriate concentrations of reagents for the unique amplification, as well as the amplification of at least one negative control and one positive control, the latter generated by transformation of E. coli cells with a fragment of the 16S rRNA gene of the amplified bacteria. Further, it allows an easy, effective, and economical alternative for monitoring sexually transmitted genital infections, such as cervicitis and urethritis. METHOD FOR OBTAINING SILVER NANOSTRUCTURES USING NEJAYOTE AS A REDUCING AGENT The present invention belongs to the field of nanotechnology, particularly to the technical field of manufacturing or treatment of nanostructures. Also, it relates to a method for obtaining silver (Ag) nanostructures, using nejayote as a reducing and stabilizing agent as a residue of the nixtamalization process for reducing silver nitrate (AgNO3, metallic precursor). Its development is in a few steps without using plants, bacteria, or agents, and it does not require sophisticated equipment that uses other resources (such as high energy consumption or high pressures) that contaminate the environment for forming Ag nanostructures. La presente invención brinda un conjunto de primers específicos, el método y su integración a un kit para la identificación única de Mycoplasma genitalium, mediante la técnica PCR utilizando los primers específicos SEQ Y SEQ 2 e incluye el uso de las concentraciones adecuadas de los reactivos para la amplificación única, así como la amplificación al menos un testigo negativo y un testigo positivo. La invención brinda un método rápido y altamente sensible para usarse con cualquier equipo de PCR, para laboratorios de biología molecular con infraestructura básica. En esta invención se incluye el uso de las concentraciones adecuadas de los reactivos para la amplificación única, así como la amplificación al menos de un control negativo y un control positivo éste último generado por transformación de células de E. coIi con un fragmento del gen 16S rRNA de la bacteria que se desea amplificar. La invención brinda un método rápido y altamente sensible para usarse con cualquier equipo de PCR, para laboratorios de biología molecular con infraestructura básica. La detección de M. genitalium mediante la presente invención, brinda una alternativa fácil, eficaz y económica para dar seguimiento a infecciones genitales de transmisión sexual como cervicitis y uretritis.