PRODUCTION OF RECOMBINANT MICROFILAMENTS AND THEIR USE AS ADJUVANTS IN SUBUNIT VACCINES

• Main Authors: Alba Adriana VALLEJO CARDONA, Rodolfo GUTIÉRREZ HERNANDEZ, Moises MARTINEZ VELAZQUEZ, Abel GUTIERREZ ORTEGA, Sara Elisa HERRERA RODRÍGUEZ, Carlos Alberto Manuel CABRERA, Lorena ZAPATA CUELLAR, Eduardo PADILLA CAMBEROS
• Format: Patent
• Language: English; Spanish
• Published: 29.05.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The present invention relates to adjuvant microfilaments. The microfilaments are VLPs that are composed of capsid proteins of the Tobacco Etch Virus labeled with 6xHis. The microfilaments of TEV-CP-His show adjuvant activity, stimulating the adaptive immune response when mixed with an antigen, which gives rise to its usefulness in the elaboration and production of a wide range of immunogenic compositions and/or for the development and production of subunit vaccines. The invention has the important attribute of biocompatibility, unlike other adjuvants, its proteic nature and origin not related to human pathogenesis favor its function by decreasing the risk of adverse effects. La presente invención se refiere a microfilamentos adyuvantes, los microfilamentos son VLPs que están compuestas por proteínas de la cápside del Virus del Grabado del Tabaco etiquetadas con 6xHis. Los microfilamentos de TEV-CP-His muestran actividad adyuvante, estimulando la respuesta inmune adaptativa cuando se mezclan con un antígeno, lo que da lugar a su utilidad en la elaboración y producción de una vasta gama composiciones inmunogénicas y/o para el desarrollo y producción de vacunas de subunidades. La invención cuenta con el importante atributo de biocompatibilidad, a diferencia de otros adyuvantes su naturaleza proteica y origen no relacionado con patogenias humanas favorecen su función disminuyendo el riesgo de efectos adversos.