METHOD FOR OBTAINING POTATO PLANTS FROM THE EXTRACTION OF INDUCED SPROUTS IN CONTROLLED CONDITIONS

• Main Authors: JOSÉ LUIS MARTÍN PÉREZ SANTOS, YOLANDA ELIZABETH MORALES GARCÍA, YAIR OLOVALDO SANTIAGO SAENZ, JESÚS MUÑOZ ROJAS, ANA LAURA HERNÁNDEZ TENORIO, VERÓNICA QUINTERO HERNÁNDEZ, ANTONIO BAEZ ROGELIO
• Format: Patent
• Language: English; Spanish
• Published: 20.04.2017
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

The present disclosure is related to a method for obtaining healthy potato seedlings inoculated with beneficial bacteria, through an economic, easy to implement and efficient process. Contemplating the sprout induction in potato tubers, under complete darkness conditions, a relative humidity (RH) of 70% and 25°C; then the stains are removed and inoculated with a mixture of three beneficial bacteria producing inhibitory substances (EMMIM-5: comprised of Gluconacetobacter diazotrophicus PA15, Pseudomonas putida KT2440 and Sphingomonas sp. OF178). The inoculated sprouts are placed in bottles with sterile vermiculite, where they grow under plant chamber conditions with a 16/8 light/dark photoperiod, at 28°C/25°C, at 80% RH. The seedling growth of inoculated sprouts is healthy and good-looking, in contrast, the seedlings of uninoculated sprouts are easily contaminated with fungi. The generated seedlings can be transplanted to larger pots under greenhouse conditions or be cultivated in the field. The method of this invention has been referred to as EIBP-EMMIM-5, that means: Extraction and inoculation of potato sprouts with the EMMIM-5 bacteria mixture. La presente invención describe como obtener plántulas de papa sanas e inoculadas con bacterias benéficas, mediante un proceso económico, fácil de llevar a cabo y eficiente. El proceso contempla la inducción de brotes en tubérculos de papa, bajo condiciones de obscuridad, 70% de humedad relativa (HR) y temperatura de 25 oC, después se extraen los brotes y son inoculados con una mezcla de tres bacterias benéficas productoras de sustancias inhibitorias (EMMIM-5: conformado por Gluconacetobacter diazotrophicus PAl5, Pseudomonas putida KT2440 y Sphingomonas sp. OF178). Los brotes inoculados se colocan en frascos con vermiculita estéril, donde crecen bajo condiciones de cámara de plantas con un fotoperiodo luz/obscuridad 16/8, a 28 oC/25 oC, a 80% HR. El crecimiento de plántulas de brotes inoculados es sano y de buena apariencia en cambio las plántulas de brotes no inoculados se contaminan fácilmente con hongos. Las plántulas generadas pueden ser trasplantadas a macetas de mayor tamaño en condiciones de invernadero o bien ser cultivadas en campo. El método de esta invención se ha denominado EIBP-EMMIM-5 y que significa "Extracción e Inoculación de brotes de papa con la mezcla de bacterias EMMIM-5".