COMPARTMENTALIZED CONTINUOUS MICRO-REACTORS FOR THE PRODUCTION OF BIOPHARMACEUTICALS IN OVARY CELLS OF CHINESE HAMSTER

The present invention refers to a group of micro-devices that a) have a plurality of cylindrical-shaped compartments obstructed by a central structure (toroidal or doughnut-type compartments); b) have a texture in the surface of the channel that promotes the cellular adherence and the retention or p...

Full description

Saved in:
Bibliographic Details
Main Authors LUCIA D. GARZA GARCIA, SANDRA OZUNA CHACON, MARIO MOISES ALVAREZ
Format Patent
LanguageEnglish
Spanish
Published 24.06.2014
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
Online AccessGet full text

Cover

More Information
Summary:The present invention refers to a group of micro-devices that a) have a plurality of cylindrical-shaped compartments obstructed by a central structure (toroidal or doughnut-type compartments); b) have a texture in the surface of the channel that promotes the cellular adherence and the retention or proliferation of the recombinant cells of the mammal, particularly but not exclusively, ovary cells of Chinese hamster; c) are fed with a culture medium in a continuous manner by some type of pumping device. This plurality of micro-devices may be used in screening applications for culturing recombinant cells of mammals, particularly but not exclusively CHO cells, or for the production of biopharmaceuticals or other recombinant proteins. La presente invención se refiere a un grupo de micro-dispositivos que (a) posean una serie de comportamientos cilíndricos obstruidos por una estructura central (compartimientos de geometría toroidal o tipo dona); (b) que posean una textura en la superficie(s) del canal que propicie la adherencia celular y la retención ó proliferación de células recombinantes de mamífero, particularmente pero no exclusivamente, células de ovario de hámster chino; y (c) que sean alimentados con medio de cultivo en forma continua por algún dispositivo de bombeo. Esta pluralidad de micro-dispositivos pueden ser empleados en aplicaciones de tamizaje de condiciones para el cultivo de células recombinantes de mamífero, particularmente pero no exclusivamente células CHO, o para la producción de biofármacos u otras proteínas recombinantes.
Bibliography:Application Number: MX20120014894