PROBES AND PRIMERS FOR DETECTION OF CHIKUNGUNYA

• Main Authors: MANJULA JAGANNATH, PILLARISETTI VENKATA SUBBARAO, CHANDRASEKHAR BHASKARAN NAIR
• Format: Patent
• Language: English; Spanish
• Published: 06.03.2012
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; DERIVATIVES THEREOF; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC CHEMISTRY; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; SUGARS; TESTING; VINEGAR; WINE

The present disclosure gives a detailed description of methods for determining the presence of Chikungunya viral nucleic acids in blood/serum/plasma samples by employing "Oligonucleotide" probes. The designed "Oligonucleotide" probes can be used for qualitative or quantitative detection of Chikungunya virus in an infected sample by employing Real time PCR. La presente invención describe un método para determinar la presencia de ácidos nucleicos de Chikungunya viral en muestras de sangre/suero o plasma mediante el empleo de sondas de "Oligonucleótidos". Las sondas designadas de "Oligonucleótidos" pueden emplearse para la detección cualitativa o cuantitativa del virus de Chikungunya en una muestra infectada mediante PCR en tiempo real.