METHOD FOR CELLULAR TISSUE MULTIPLICATION FROM JATROPHA CURCAS

• Main Authors: SANDRA MARCELA CORREA CORDOBA, LUCIA ATEHORTUA GARCES
• Format: Patent
• Language: English; Spanish
• Published: 20.10.2010
• Subjects: ANIMAL AND VEGETABLE OILS, FATS, FATTY SUBSTANCES AND WAXES; BEER; BIOCHEMISTRY; CANDLES; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; DETERGENTS; ENZYMOLOGY; ESSENTIAL OILS; FATTY ACIDS THEREFROM; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PERFUMES; PRODUCING (PRESSING, EXTRACTION), REFINING AND PRESERVINGFATS, FATTY SUBSTANCES (e.g. LANOLIN), FATTY OILS AND WAXES,INCLUDING EXTRACTION FROM WASTE MATERIALS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The method of the present invention comprises: Obtaining an explant from the seeds of Jatropha curcas; Putting the explant derived from the seed of Jatropha curcas in a culture medium; Breaking the intercellular unions of the explants tissue, which generates individuals cells; Incubating for a determined time the culture medium with the generated individual cells, that were multiplied; and, Extracting oil from the cells that multiplied from the individual cells generated from the explants derived from the Jatropha curcas seed. El método de la presente invención comprende: Obtener un explante de la semillas de Jatropha curcas; Poner el explante derivado de la semilla de Jatropha curcas en un medio de cultivo; Romper las uniones intercelulares del tejido de los explantes, los que generan células individuales; Incubar por un tiempo determinado el medio de cultivo con las células individuales generadas, las cuales se multiplican; y, Extraer aceite de las células que se multiplicaron a partir de las células individuales generadas de los explantes derivados de la semilla de Jatropha curcas.