DETECTION AND TYPIFICATION O 20 TYPES OF HPV BY POLYMERASE CHAIN REACTION (PCR) IN MULTIPLEX FORMAT
The present invention refers to a method for detecting and typifying 20 types of HPV, mainly to a method by polymerase chain reaction (PCR) in multiplex format. The novel design for diagnosing HPV by multiplex PCR, allows, in a reduced number of reactions, to identify the presence and the type of HV...
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Main Author | |
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Format | Patent |
Language | English Spanish |
Published |
23.06.2010
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Subjects | |
Online Access | Get full text |
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Summary: | The present invention refers to a method for detecting and typifying 20 types of HPV, mainly to a method by polymerase chain reaction (PCR) in multiplex format. The novel design for diagnosing HPV by multiplex PCR, allows, in a reduced number of reactions, to identify the presence and the type of HVP, being only based on their amplification pattern. The identity of the amplified bands is deduced by the localization thereof in the reaction tubes and by the size of the bands amplified by agarose gel electrophoresis. In addition, the bands identity may be confirmed by means of the pattern produced by restriction enzymes digestion.
La presente invención es relativa a un método de detección y tipificación de 20 tipos de Virus de Papiloma Humano (VPH), principalmente a un método por reacción en cadena de la polimerasa en formato múltiplex. El nuevo diseño de diagnóstico de VPH con PCR-multiplex, permite que en un número reducido de reacciones, se identifiquen la presencia y el tipo de VPH basados únicamente en su patrón de amplificación. La identidad de las bandas amplificadas se deduce por su localización en los tubos de reacción y por el tamaño de las bandas amplificadas por electroforesis en un gel de agarosa. Adicionalmente, se puede confirmar la identidad de las bandas por medio del patrón producido por digestión con enzimas de restricción. |
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Bibliography: | Application Number: MX20090000664 |