MONOCOT AHASS SEQUENCES AND METHODS OF USE

• Main Authors: GREGORY BUDZISZEWSKI, GENICHI KAKEFUDA, ROBERT A. ASCENZI, BIJAY K. SINGH
• Format: Patent
• Language: English; Spanish
• Published: 11.03.2008
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; DERIVATIVES THEREOF; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC CHEMISTRY; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; SUGARS; TRAPPING; VINEGAR; WINE

Isolated polynucleotides that encode acetohydroxyacid synthase small subunit (AHASS) polypeptides, and the amino acid sequences encoded by these polynucleotides, are described. The present invention provides expression cassettes and plant expression vectors comprising the polynucleotides encoding the AHASS polypeptides or AHASS fusion polypeptides. Also provided are plants, seeds, and host cells transformed with the polynucleotides, expression cassettes, or expression vectors of the present invention. The invention further provides methods of using the polynucleotides of the present invention to enhance the AHAS activity and to enhance tolerance of plants to herbicides. La presente invencion se refiere a polinucleotidos aislados que codifican polipeptidos de subunidad pequena de sintasa acetohidroxiacida (AHASS), y las secuencias de aminoacidos codificadas por estos polinucleotidos. La presente invencion provee casetes de expresion y vectores de expresion en plantas que comprenden los polinucleotidos que codifican los polipeptidos AHASS o polipeptidos de fusion AHASS, Ademas se proveen plantas, semillas, y celulas hospedadoras transformadas con los polinucleotidos, casetes de presion, o vectores de expresion de la presente invencion. La invencion ademas provee metodos de uso de los polinucleotidos de la presente invencion para mejorar la actividad de la presente invencion para mejorar la actividad de AHAS y mejorar la tolerancia de las plantas herbicidas.