Process for in vitro micropropagation of Stevia rebaudiana Bertoni

• Main Authors: CHINTEA PAVEL, COTENCO EUGENIA, SVET STEPAN, SIROMEATNICOV IULIA, JACOTA ANATOL
• Format: Patent
• Language: English; Romanian
• Published: 31.08.2009
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

The invention refers to biotechnology, particularly to a process for in vitro micropropagation of Stevia rebaudiana Bertoni plants. The process, according to the invention, includes obtaining of explants, sterilization, segmentation and cultivation thereof on the Murashige-Skoog nutrient medium, containing additionally steroid glycosides obtained from roots of Solanum melongena L. egg-plants by extraction with boiling water, treatment of the obtained extract with butanol-1 and concentration, in a concentration of (2.5...3.0)À10-3%, at the same time cultivation is carried out at a temperature of 22...26?C, air relative humidity of 70%, with a photoperiod of 16 and 18 hours day and night and the light intensity of 3000 lx. The result of the invention consists in increasing the somatic embryogenesis and regeneration of plants. Inventia se refera la biotehnologie, in particular la un procedeu de micropropagare a plantelor de Stevia rebaudiana Bertoni in vitro. Procedeul, conform inventiei, include obtinerea explantelor, sterilizarea, segmentarea si cultivarea lor pemediul nutritiv Murashige-Skoog, ce contine suplimentar glicozide steroidice obtinute din radacini de patlagele vinete Solanum melongena L. prin extractie cu apa la fierbere, prelucrarea extractului obtinut cu butanol-1 si concentrare, in concentratie de (2,5?.3,0)·10-3%, totodata cultivarea se efectueaza la o temperatura de 22?26°C, umiditatea relativa a aerului de 70%, cu o fotoperioada de 16 si 8 ore zi si noapte si intensitatea iluminarii de 3000 lx. Rezultatul inventiei consta in sporirea embriogenezei somatice si regenerarii plantelor.