Process for micropropagation in vitro of Echinacea purpurea L. Moench

• Main Authors: JACOTĂ Anatolie, SÎROMEATNICOV Iulia, ŞVEŢ Stepan, CHINTEA Pavel, COTENCO Eugenia
• Format: Patent
• Language: English; Romanian; Russian
• Published: 31.01.2010
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

The invention refers to biotechnology, particularly to a process for micropropagation in vitro of Echinacea purpurea L. Moench plants.The process, according to the invention, includes the sterile germination of seeds, obtaining of explants, segmentation and cultivation thereof on the Murashige-Skoog nutrient medium, containing additionally steroid glycosides obtained from Solanum melongena L. egg-plant roots by extraction with boiling water, treatment of the obtained extract with butanol-1 and concentration, in a concentration of (3,0...3,5)·10-3%, at the same time cultivation is carried out at a temperature of 22...26°C, air relative humidity of 70%, with a photoperiod of 16 and 18 hours day/night and illumination intensity of 3000 lx.The result of the invention consists in increasing the somatic embryogenesis and regeneration of plants. Invenţia se referă la biotehnologie, în particular la un procedeu de micropropagare a plantelor de Echinacea purpurea L. Moench in vitro.Procedeul, conform invenţiei, include germinarea aseptică a seminţelor, obţinerea explantelor, segmentarea şi cultivarea lor pe mediul nutritiv Murashige-Skoog, ce conţine suplimentar glicozide steroidice obţinute din rădăcini de pătlăgele vinete Solanum melongena L. prin extracţie cu apă la fierbere, prelucrarea extractului obţinut cu butanol-1 şi concentrare, în concentraţie de (3,0...3,5)·10-3%, totodată cultivarea se efectuează la o temperatură de 22...26°C, umiditatea relativă a aerului de 70%, cu o fotoperioadă de 16 şi 8 ore noapte/zi şi intensitatea iluminării de 3000 lx.Rezultatul invenţiei constă în sporirea embriogenezei somatice şi regenerării plantelor. Изобретение относится к биотехнологии, в частности к способу микроразмножения растений Echinacea purpurea L. Moench in vitro.Способ, согласно изобретению, включает стерильное проращивание семян, получение эксплантов, сегментацию и культивирование их на питательной среде Мурасиге-Скуга, содержащей дополнительно стероидные гликозиды, полученные из корней баклажанов Solanum melongena L. путем экстрагирования кипящей водой, обработки полученного экстракта бутанолом-1 и концентрирования, в концентрации (3,0...3,5)·10-3%, при этом культивирование ведут при температуре 22...26°С, относительной влажности воздуха 70%, фотопериоде 16 и 8 часов день/ночь и интенсивности освещения 3000 люкс.Результат изобретения состоит в увеличении соматического эмбриогенеза и регенерации растений.