DIFFERENTIAL EXPRESSION GENE OF CYNODON DACTYLON UNDER ALUMINUM STRESS AND MINING AND ANALYSIS METHOD THEREOF

• Main Authors: DUAN Hongli, HUANG Chunqiong, LIU Pandao, HE Xiao, ZHANG Yu, JIANG Lingyan, CHEN Zhijian, HUAN Hengfu
• Format: Patent
• Language: English
• Published: 21.05.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to the technical field of bioinformatics, in particular to a differentially expressed gene of Cynodon dactylon under aluminum stress, a mining and analysis method thereof. The method includes: S1, pre-culturing Cynodon dactylon; S2, treating Cynodon dactylon under aluminum stress; S3, sequencing transcriptome of Cynodon dactylon, including transcriptome original data analysis, GO enrichment analysis of differential genes and qRT-PCR analysis. According to the invention, 17 differentially expressed genes of Cynodon dactylon under aluminum stress conditions are obtained through pre-culture of Cynodon dactylon, aluminum stress treatment of Cynodon dactylon, original data analysis of root transcription group of Cynodon dactylon, GO enrichment analysis and qRT-PCR analysis of differential genes, the differentially expressed genes of Cynodon dactylon under aluminum stress conditions are excavated and analyzed for the first time.