PROCESS FOR PRODUCTION OF C-TERMINAL ALPHA-AMIDATED PEPTIDE
Processes for the production of a peptide of the formula (III): R-X-NH2, wherein X-NH2 represents a C-terminal alpha -amidated amino acid and R represents a remaining portion of the peptide, starting form any step in a series of the following steps: (1) obtaining a fused protein of the formula (0):...
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Main Authors | , , |
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Format | Patent |
Language | English Korean |
Published |
09.10.1996
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Edition | 6 |
Subjects | |
Online Access | Get full text |
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Summary: | Processes for the production of a peptide of the formula (III): R-X-NH2, wherein X-NH2 represents a C-terminal alpha -amidated amino acid and R represents a remaining portion of the peptide, starting form any step in a series of the following steps: (1) obtaining a fused protein of the formula (0): P-R-X-Gly-B, wherein P represents a partner protein of the fused protein, X represents any amino acid residue, Gly represents a glycine residue, B represents a C-terminal basic amino acid or a basic oligopeptide, and R represents a remaining portion of the peptide, by a gene recombination method; (2) recovering the fused protein; (3) cleaving the fused protein to form a partner protein (P) and a peptide of the formula (I): R-X-Gly-B; (4) separating the peptide (I) from the partner protein (P) on the basis of the difference of the isoelectric points thereof; (5) hydrolyzing the peptide (I) with carboxypeptidase B to form an intermediate peptide of the formula (II) R-X-Gly; and (6) treating the peptide (II) with a C-terminal alpha -amidating enzyme. |
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Bibliography: | Application Number: KR19900702063 |