METHOD FOR PROMOTING DISULFIDE BOND FORMATION IN RECOMBINANT PROTEINS

Reduced cysteine-containing proteins consisting of recombinant IFN-β, IL-2 muteins thereof may be oxidized selectively so that the recombinant proteins have essentially the same disulfide bridging and biological activity as their native counterparts. The oxidized product is substantially free of unw...

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Bibliographic Details
Main Authors HALENBECK, ROBERT F, COURSE, KEYSTON E
Format Patent
LanguageEnglish
Korean
Published 22.04.1994
Edition5
Subjects
CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY
CHEMISTRY
ELECTRIC COMMUNICATION TECHNIQUE
ELECTRICITY
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
PERFORMING OPERATIONS
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
PICTORIAL COMMUNICATION, e.g. TELEVISION
THEIR RELEVANT APPARATUS
TRANSPORTING
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Summary:Reduced cysteine-containing proteins consisting of recombinant IFN-β, IL-2 muteins thereof may be oxidized selectively so that the recombinant proteins have essentially the same disulfide bridging and biological activity as their native counterparts. The oxidized product is substantially free of unwanted side products and contains a minimal amount of intermolecular oligomers. The oxidation takes place in an aqueous medium containing a solubilizing agent at a pH of about 5.5 to 9, preferably at a pH of about 7. The reaction is initiated by addition of at least an effective amount of an oxidation promoter containing a Cu+2 cation such as CuCI2 or o-phenanthroline/Cu+2 complex in the presence of air.
Bibliography:Application Number: KR19850008860