METHOD FOR PREPARATION OF CYCLOMALTO DEXTRIN GLUCANO TRANSFERASE

A cyclomaltodextrin gluconotransferase (CGTase) with heat tolerance is prepd. by (a) fermenting Bacillus stearothermophilus KCTC 8495P at 55 deg.C and pH 7.0 in the medium contg. soluble starch as a carbon source, non-fat soybean residue, NaH2PO42H2O and CaCl2, (b) centrifuging the fermented broth t...

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Bibliographic Details
Main Authors HWANG, JIN - BONG, KIM, SUNG - HO
Format Patent
LanguageEnglish
Published 06.08.1992
Edition5
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:A cyclomaltodextrin gluconotransferase (CGTase) with heat tolerance is prepd. by (a) fermenting Bacillus stearothermophilus KCTC 8495P at 55 deg.C and pH 7.0 in the medium contg. soluble starch as a carbon source, non-fat soybean residue, NaH2PO42H2O and CaCl2, (b) centrifuging the fermented broth to obtain the supernatant, (c) purifying the supernatant by salting-out with ammonium sulfate and affinity chromatography, and (d) dialysing the obtd. soln in the D.W.. The obtd. CGTase is useful for producing cyclodextrin cpds. from starch.
Bibliography:Application Number: KR19900013443