IMPROVING METHOD OF ENZYME REACTION

L-Tryptophan is prepd. from L- or DL-serine, indole, and ammonium salts enzymatically in nonaqueous solvent systems. Thus, a mixt. of L-serine 9.27, (NH4)2 SO4 3, pyridoxal phosphate 0.01 and H2O 82.5g is adjusted to pH8.5, mixed with E. coli MT10242 cream cake 3 and acetyltributyl citrate (contg, i...

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Main Authors OOOKA MASAHARU, NAGAI SHOUSUKE, DAKANO DAKAO, YOSHIGAWA YUKIHIRO, KAYASHIMA NOBUYUKI, GAWASHIMA NOBUHIRO
Format Patent
LanguageEnglish
Korean
Published 11.06.1987
Edition4
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:L-Tryptophan is prepd. from L- or DL-serine, indole, and ammonium salts enzymatically in nonaqueous solvent systems. Thus, a mixt. of L-serine 9.27, (NH4)2 SO4 3, pyridoxal phosphate 0.01 and H2O 82.5g is adjusted to pH8.5, mixed with E. coli MT10242 cream cake 3 and acetyltributyl citrate (contg, indole) 68.9g. The reaction mixt. is incubated at 35 C for 2hr to give 96.5% Ltryptophan based on the indole used. Nonaqueous onganic solvent contains toluene, methyl iso- ketone, diisoketone, diiso butyl ketone, ethyl citrate, and benzene.
Bibliography:Application Number: KR19830006285