A METHOD FOR STABILIZING A TUMOR NECTROSIS FACTOR

A tumor necrosis factor(TNF) compn. was stabilized in aq. soln. or powder form by the addn. of at least one stabilizer(I) selected from albumin, gelation, protamines and protamine salts. Thus, a soln. of rabbit TNF(sp. activity; 5.0 x105 u/mg) in 0.15M NaCl was dild. with 0.1M phosphate buffer(pH 7....

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Bibliographic Details
Main Authors SAKAMOTO HAJIMU, HAYASHI HIROSHI, KIYOTA TAKAO
Format Patent
LanguageEnglish
Korean
Published 09.07.1986
Edition4
Subjects
HUMAN NECESSITIES
HYGIENE
MEDICAL OR VETERINARY SCIENCE
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
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Summary:A tumor necrosis factor(TNF) compn. was stabilized in aq. soln. or powder form by the addn. of at least one stabilizer(I) selected from albumin, gelation, protamines and protamine salts. Thus, a soln. of rabbit TNF(sp. activity; 5.0 x105 u/mg) in 0.15M NaCl was dild. with 0.1M phosphate buffer(pH 7.0) to form a TMF soln. with an activity of 1200 u/ml. HSA, BSA, HGG, and PS were sep. added to portion of TNF soln. to form 2 different solns(samples) having a concn. of 0.1 mg/ml or 1.0 mg/ml. The residual activity of the sample with a (I) was 87 - 100% after treatments while that of the control TNF sample without a (I) was 4 - 52%. (I) stabilizes TNF against loss of activity during storage, freeze-drying and freeze-thaw treatment. The stabilized soln. may be freeze dried to obtain compns. on powder form.
Bibliography:Application Number: KR19830001415