CRISPR/Cas CRISPR/CAS Screening Platform To Identify Genetic Modifiers Of Tau Seeding Or Aggregation

• Main Authors: KOSS MATTHEW, ZAMBROWICZ BRIAN, FURY WEN, PRISSETTE MARINE
• Format: Patent
• Language: English; Korean
• Published: 20.08.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

Cas-단백질-대응(ready) 타우(tau) 바이오센서 세포, CRISPR/Cas 상승작용적 활성화 매개자(SAM: synergistic activation mediator)-대응 타우 바이오센서 세포, 및 타우 시딩(seeding) 또는 응집의 유전적 변형제를 스크리닝하기 위해 이러한 세포를 제조하는 방법 및 사용하는 방법이 제공된다. 이러한 세포를 타우 시딩 활성 또는 타우 응집에 대해 민감화시키거나 타우 응집을 야기하기 위한 시약 및 방법이 또한 제공된다. Cas-protein-ready tau biosensor cells, CRISPR/Cas synergistic activation mediator (SAM)-ready tau biosensor cells, and methods of making and using such cells to screen for genetic modifiers of tau seeding or aggregation are provided. Reagents and methods for sensitizing such cells to tau seeding activity or tau aggregation or for causing tau aggregation are also provided.