ENZYMATIC CIRCUITS FOR MOLECULAR SENSORS

• Main Authors: MOLA PAUL, GOVINDARAJ VENKATESH ALAGARSWAMY, MERRIMAN BARRY L, GEISER TIM
• Format: Patent
• Language: English; Korean
• Published: 12.08.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TESTING; VINEGAR; WINE

여러 실시형태들에서, 분자 회로가 개시된다. 분자 회로는 음의 전극; 음의 전극으로부터 이격되어 있는 양의 전극; 및 양의 전극 및 음의 전극 양쪽에 전도적으로 부착되어 효소를 통한 전도 경로를 형성하는 효소 분자를 포함한다. 여러 예들에서, 효소는 폴리메라제이다. 회로는 효소를 전극들에 배선하는데 사용되는 분자 아암을 더 포함할 수 있다. 여러 실시형태들에서, 회로는 센서로서 기능하며, 회로에서 전기신호들, 이를 테면, 전압, 전류, 임피던스, 컨덕턴스 또는 저항의 변화는 기질이 효소와 상호 작용할 때 측정된다. In various embodiments a molecular circuit is disclosed. The circuit comprises a negative electrode, a positive electrode spaced apart from the negative electrode, and an enzyme molecule conductively attached to both the positive and negative electrodes to form a circuit having a conduction pathway through the enzyme. In various examples, the enzyme is a polymerase. The circuit may further comprise molecular arms used to wire the enzyme to the electrodes. In various embodiments, the circuit functions as a sensor, wherein electrical signals, such as changes to voltage, current, impedance, conductance, or resistance in the circuit, are measured as substrates interact with the enzyme.