Purification method of botulinum toxin

• Main Authors: YUN BUM JIN, SHIM MYUNGBO, KIM JAE YOUNG, NAM JEONG SUN, KIM SEUNGHO, KIM MINJOONG, KIM JOOYEON
• Format: Patent
• Language: English; Korean
• Published: 16.05.2024
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

본 발명은 보툴리눔 독소(Botulinum Toxin, BTX)의 정제방법에 관한 것으로, 더 상세하게는 양이온 교환 크로마토그래피(Cation Exchange Chromatography; CEX), 소수성 크로마토그래피(Hydrophobic Interaction Chromatography; HIC) 및 혼합 모드 크로마토그래피(Mixed Mode Chromatography: Cation Exchange(Phosphate) and Affinity(Calcium))를 이용하여 정제하는 단계의 순서로 수행하되, 보툴리눔 독소를 활성화시키기 위해 트립신(Trypsin)을 처리하는 단계를 추가로 포함하는 공정으로 순도 및 활성이 뛰어난 보툴리눔 독소를 정제할 수 있어, 보툴리눔 독소 생산에 유용하게 활용될 수 있다. The present invention relates to a method for purifying botulinum toxin (BTX). More specifically, the method is performed in the order of purification steps using cation exchange chromatography (CEX), hydrophobic interaction chromatography (HIC), and mixed mode chromatography (cation exchange (phosphate) and affinity (calcium)), the method further comprising the step of treating trypsin to activate the botulinum toxin. Therefore, the method can purify botulinum toxin having excellent purity and activity and thus can be useful in producing botulinum toxin.