신규 방법

• Main Authors: NUSSBAUMER OLIVER, HUTTON ANDREW JOHN, KOVACS ISTVAN
• Format: Patent
• Language: Korean
• Published: 07.12.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

본 발명은 γδ T 세포가 농축된 조성물을 제조하는 단계 및 영양세포의 존재 하에 조성물을 배양하는 단계를 포함하는 γδ T 세포를 증식시키는 방법에 관한 것이다. 또한, γδ T 세포가 농축된 조성물을 제조하는 단계, 조성물을 종양 관련 항원에 특이적인 키메라 항원 수용체(CAR)를 발현하도록 형질도입하는 단계, 및 형질도입된 조성물을 배양하여 조작된 γδ T 세포를 증식시키는 단계를 포함하는 γδ T 세포를 조작하는 방법이 제공된다. 또한, 차용 T 세포 치료법, 키메라 수용체 치료법 등에서 유용성을 확인하는, 기재된 방법에 따라 제조된 증식되고 조작된 γδ T 세포가 제공된다. The invention relates to methods for expanding γδ T cells comprising preparing a composition enriched for γδ T cells and culturing the composition in the presence of feeder cells. Also provided is a method for engineering γδ T cells comprising preparing a composition enriched for γδ T cells, transducing the composition to express a chimeric antigen receptor (CAR) specific for a tumour associated antigen and culturing the transduced composition to expand the engineered γδ T cells. Also provided are expanded and engineered γδ T cells produced according to the described methods, which find utility in adoptive T cell therapies, chimeric receptor therapies and the like.