MULTIVALENT BINDING COMPOSITION FOR NUCLEIC ACID ANALYSIS

• Main Authors: LEVIEUX JAKE, LOPEZ TYLER, PREVITE MICHAEL, ZHAO JUNHUA, HE MOLLY, ARSLAN SINAN, ZHANG SU, KELLINGER MATTHEW
• Format: Patent
• Language: English; Korean
• Published: 05.12.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

입자에 부착된 뉴클레오타이드의 복수의 카피를 가지는 입자-뉴클레오타이드 컨쥬게이트를 포함하는 다가 결합 조성물이 기재된다. 다가 결합 조성물은 검출가능한 신호를 생화학적 상호작용의 활성 영역, 예를 들어, 단백질-단백질 상호작용, 단백질-핵산 상호작용, 핵산 혼성화, 또는 효소 반응의 부위에 국소화할 수 있도록 하며, 폴리머라제 반응 동안 연장되는 핵산 사슬 내 염기 혼입 부위를 확인하고 시퀀싱 및 어레이 기반 적용을 위한 개선된 염기 식별을 제공하기 위해 사용될 수 있다. Multivalent binding compositions including a particle-nucleotide conjugate having a plurality of copies of a nucleotide attached to the particle are described. The multivalent binding compositions allow one to localize detectable signals to active regions of biochemical interaction, e.g., sites of protein-protein interaction, protein-nucleic acid interaction, nucleic acid hybridization, or enzymatic reaction, and can be used to identify sites of base incorporation in elongating nucleic acid chains during polymerase reactions and to provide improved base discrimination for sequencing and array based applications.