시토크롬 P450 효소 돌연변이체 및 이의 응용

• Main Authors: CHENG YIBING, JAMES GAGE, HONG HAO, MA YULEI, CAO SHAN, ZHANG NA, JIAO XUECHENG, SUN KAIHUA
• Format: Patent
• Language: Korean
• Published: 10.10.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

시토크롬 P450 효소 돌연변이체 및 이의 응용을 제공한다. 유도 진화의 수단을 통해 바실러스 메가테리움(Bacillus megaterium) 야생형 균주에서 유래된 P450의 효소 활성과 안티 마르코프니코프 산화 선택성에 대해 단백질 변형을 수행하여 효소 활성 및 선택성을 향상시키고 산업화 생산에 사용될 수 있는 일련의 P450 효소 돌연변이체를 개발하였다. Provided are a cytochrome P450 enzyme mutant and an application thereof. The enzyme activity and anti-Markov oxidation selectivity of P450 derived from a wild-type strain of Bacillus megaterium undergo protein modification by means of directed evolution, thus improving enzyme activity and selectivity, and developing a series of P450 enzyme mutants that may be used for industrial production.