신규한 곤충 저해 단백질

• Main Authors: CICHE TODD A, WALDHEUSER STEPHANIE C, BOWEN DAVID J, HOWE ARLENE R, WEGENER KIMBERLY M
• Format: Patent
• Language: Korean
• Published: 31.08.2023
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS; PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

나비목해충 종에 대하여 독성 활성을 나타내는 살균살충 단백질이 개시되고, 그리고 TIC13085 및 TIC13087을 포함하지만 이들에 한정되지 않는다. 개시된 살균살충 단백질 중에서 하나 이상을 인코딩하는 재조합 핵산 서열을 내포하는 DNA 작제물이 제공된다. 나비목 침입에 대해 내성인 유전자도입 식물, 식물 세포, 종자 및 식물 부분이 제공되는데, 이들은 본원 발명의 살균살충 단백질을 인코딩하는 재조합 핵산 서열을 내포한다. 생물학적 샘플 내에서 본원 발명의 재조합 핵산 서열 또는 단백질의 존재를 검출하기 위한 방법, 그리고 TIC13085 및 TIC13087 살균살충 단백질 중 임의의 것을 이용하여 나비목종 해충을 제어하는 방법 역시 제공된다. Pesticidal proteins exhibiting toxic activity against Lepidopteran pest species are disclosed, and include, but are not limited to, TIC13085 and TIC13087. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding one or more of the disclosed pesticidal proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Lepidopteran infestation are provided which contain recombinant nucleic acid sequences encoding the pesticidal proteins of the present invention. Methods for detecting the presence of the recombinant nucleic acid sequences or the proteins of the present invention in a biological sample, and methods of controlling Lepidopteran species pests using any of the TIC13085 and TIC13087 pesticidal proteins are also provided.