비드-연결된 트랜스포좀을 사용한 RNA 및 DNA 시퀀싱 라이브러리의 제작

• Main Authors: KHURANA TARUN, RANDISE HINCHLIFF CARLO, SCHROTH GARY, GORMLEY NIALL ANTHONY, WU YIR SHYUAN, KUERSTEN ROBERT SCOTT, KENNEDY ANDREW B, MASHAYEKHI FOAD, KAPER FIONA, SHULTZABERGER SARAH
• Format: Patent
• Language: Korean
• Published: 24.03.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

본 출원은 태그화 RNA 단편들의 고정된 라이브러리를 제작하는 방법을 설명한다. 또한, 단일 샘플로부터 DNA 및 RNA 시퀀싱 라이브러리를 제작하는 다수의 방법이 본원에 기재된다. 이들 방법은 단일 세포로부터의 라이브러리 제작을 포함할 수 있다. This application describes methods of preparing an immobilized library of tagged RNA fragments. Also described herein are a number of methods of preparing DNA and RNA sequencing libraries from a single sample. These methods can include library preparation from single cells.