C/D C Vaccine Composition Comprising Recombinant Protein Derived from Heavy-Chain C-terminal Domain of Clostridium Botulinum Type C/D

• Main Authors: SON YOUNG MIN, LEE KYUNG HYUN, HWANG MI HYE, JUNG BYEONG YEAL, YOON SOON SEEK
• Format: Patent
• Language: English; Korean
• Published: 09.03.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; TESTING; VINEGAR; WINE

The present invention relates to a recombinant vector containing a gene encoding heavy-chain C-terminal proteins of botulinum type C/D derived from botulinum type C/D strains, a vaccine composition for preventing botulism type C/D addiction of livestock, containing proteins derived from heavy-chain C-terminal of botulinum C/D type, and a composition for diagnosing botulism C/D type addiction of livestock, containing recombinant proteins derived from heavy-chain C-terminal of botulinum C/D type. The present invention uses transgenic E.coli, which produces recombinant proteins derived from heavy-chain C-terminals of botulinum toxin type C/D in large quantity, to make use of heavy-chain C-terminal proteins of botulinum toxin type C/D generated by the strains as an antibody for vaccine and diagnosis, thereby effectively preventing and diagnosing botulism type C/D generated in livestock, expecting that livestock mortality rates will be greatly reduced. 본 발명은 보툴리눔 C/D형 균주로부터 유래된 보툴리눔 C/D형의 중쇄 C말단 단백질을 인코딩하는 유전자를 포함하는 재조합 벡터, 보툴리눔 C/D형 중쇄 C말단 유래 재조합 단백질을 포함하는 가축의 보툴리즘 C/D형 중독증 예방용 백신 조성물 및 보툴리눔 C/D형 중쇄 C말단 유래 재조합 단백질을 포함하는 가축의 보툴리즘 C/D형 중독증 진단용 조성물에 관한 것이다. 본 발명은 본 발명은 보툴리눔 독소 C/D형 중쇄 C말단 재조합 단백질을 대량 생산하는 형질전환 대장균을 이용하여 상기 균주가 생성하는 보툴리눔 독소 C/D형 중쇄 C말단 단백질을 백신 및 진단용 항원으로 사용함으로써, 가축에서 발생하는 C/D형 보툴리즘을 효과적으로 예방 및 진단하여 가축의 폐사율을 크게 감소시킬 것으로 예상된다.