T miRNA Composition comprising miRNA derived from extracellular vesicles of T cells as an active ingredient and use thereof

• Main Authors: YEA KYUNG MOO, JUNG DOKYUNG, BAEK MOON CHANG
• Format: Patent
• Language: English; Korean
• Published: 16.02.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The present invention relates to a composition containing T cell extracellular vesicle-derived miRNA as an active ingredient and uses thereof. In T cells transfected with vectors containing IL-2, linker, and transmembrane proteins, extracellular vesicles expressing the IL-2 are produced on surfaces. Of the miRNAs contained in the extracellular vesicles that express the IL-2 on the surfaces, mir-101-3p, miR-181a-3p, miR-223-3p, miR-619-5p, miR-1246, miR-3182, miR-4787-5p, and miR-5787 exhibit anticancer activity by suppressing the expression of PD-L1 and Rab 27a genes in cancer cells. It was confirmed that miR-17-5p, miR-29a-3p, miR-92a-1-5p, miR-125a-5p, miR-181a-3p, and miR-223-3p exhibit an immune-promoting effect by increasing the expression levels of Ki-67, IFN-γ, and Granzyme B genes in the T cells. Compositions containing the above-described miRNAs are provided as anticancer compositions and immune-promoting compositions. 본 발명은 T 세포의 세포외 소포체 유래 miRNA를 유효성분으로 포함하는 조성물 및 이의 용도에 관한 것으로, IL-2, 링커 및 막관통 단백질을 포함하는 벡터로 형질감염된 T 세포에서는 표면에 IL-2를 발현하는 세포외 소포체가 생산되고, IL-2를 표면에 발현하는 세포외 소포체에 함유된 miRNA들 중에서 miR-101-3p, miR-181a-3p, miR-223-3p, miR-619-5p, miR-1246, miR-3182, miR-4787-5p 및 miR-5787은 암세포의 PD-L1 및 Rab 27a 유전자의 발현을 억제하여 항암 활성을 나타내고, miR-17-5p, miR-29a-3p, miR-92a-1-5p, miR-125a-5p, miR-181a-3p 및 miR-223-3p는 T 세포의 Ki-67, IFN-γ 및 Granzyme B 유전자의 발현 수준을 증가시키는 면역 증진 효과를 나타냄을 확인함으로써, 상기 miRNA들을 포함하는 조성물은 항암용 조성물 및 면역증진용 조성물로 제공된다.