1- MBLB1156BACILLUS ATROPHAEUS MBLB1156 COMPRISING 1-DEOXYNOJIRIMYCIN BIOSYNTHETIC GENE CLUSTER

In the present invention, 1-DNJ core biosynthesis gene group was isolated from Bacillus atrophaeus MBLB1156 (KCCM12922P) isolated from fermented foods and characteristics thereof were studied through the heterozygous expression of each gene. First, GabT1, a first biosynthetic enzyme, was found to pr...

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Bibliographic Details
Main Authors CHUN JEONGHYE, SEO MYUNG JI
Format Patent
LanguageEnglish
Korean
Published 26.07.2022
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:In the present invention, 1-DNJ core biosynthesis gene group was isolated from Bacillus atrophaeus MBLB1156 (KCCM12922P) isolated from fermented foods and characteristics thereof were studied through the heterozygous expression of each gene. First, GabT1, a first biosynthetic enzyme, was found to prefer fructose-6-phosphate as an amino group acceptor and L-glutamic acid as an amino group donor as an aminotransferase in the presence of the coenzyme PLP. Yktc1, a second biosynthetic enzyme, was found to catalyze the dephosphorylation reaction with a zinc ion-dependent phosphatase, and the optimal activity condition was 65 ℃ and pH 5.0. In addition, Yktc1 was found to have a wide range of substrate specificities. GutB1, the last biosynthetic enzyme, has a high substrate specificity for 2-amino-2-deoxy-D-mannitol, and the optimum activity condition was found to be 30 ℃ and pH 9.5. The characteristic analysis of biosynthetic enzymes performed in the present invention can be used as basic data for identifying and understanding the biosynthetic pathway of 1-DNJ. 본 발명은 발효식품에서 분리된바실러스 아트로페어스 MBLB1156 (Bacillus atrophaeus MBLB1156)(KCCM12922P)으로부터 1-DNJ 핵심 생합성 유전자 군을 분리하여 각 유전자들의 이형발현을 통해 특성을 연구하였다. 먼저 첫 번째 생합성 효소인 GabT1은 조효소인 PLP 존재하에 아미노기전이효소로 아미노기 공여체로 L-글루탐산을 아미노기 수용체로 fructose-6-phosphate를 선호하는 것으로 확인되었다. 두 번째 생합성 효소 Yktc1은 zinc이온 의존성 포스파타아제로 탈인산화반응을 촉매하는 것으로 확인이 되었고 최적활성 조건은 65℃ pH 5.0 였다. 또한 Yktc1은 넓은 범위의 기질 특이성을 갖는 것으로 확인되었다. 마지막 생합성 효소 GutB1은 2-amino-2-deoxy-D-mannitol에 대해 높은 기질 특이성을 가지고 최적활성 조건은 30℃ pH 9.5로 확인되었다.
Bibliography:Application Number: KR20210007459