Reverse transcriptase nested polymerase chain reaction for the detection of Aichivirus-A from water environment and positive control for confirmation of the false positive reaction

• Main Authors: JOO YOUNLEE, LEE SIWON, LEE JIN YOUNG, BAE KYUNGSEON, KIM JIHYE, YOU KYUNG A, CHUNG HYEN MI, LEE SOO HYUNG
• Format: Patent
• Language: English; Korean
• Published: 19.04.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The present invention provides a primer set for detecting Aichivirus-A (AiV-A), which can rapidly confirm AiV-A causing acute gastroenteritis in humans with high specificity and detection sensitivity, a detection kit, a detection method, a positive control group capable of distinguishing a false positive reaction, and uses thereof. 본 발명은 사람에게 급성 위장염을 일으킬 수 있는 아이치바이러스-A(Aichivirus-A; AiV-A)를 높은 특이도 및 검출 민감도로 신속하게 확인할 수 있는 아이치바이러스-A 검출용 프라이머 세트, 검출 키트 및 검출 방법과 위양성 반응을 구분할 수 있는 양성대조군 및 이의 용도에 관한 것이다.