II Engineered Angiotensin Converting Enzyme II and Uses Thereof

• Main Authors: LEE SEUNGHYUN, CHOI JIN KYUNG, IM SUN WOO, HWANG BORA, KWON BYOUNG S
• Format: Patent
• Language: English; Korean
• Published: 09.12.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The present invention relates to an angiotensin converting enzyme II (ACE2) mutant with high coronavirus binding affinity and neutralization ability, fusion protein including the same, and uses thereof for preventing or treating coronavirus. The angiotensin converting enzyme II (ACE2) mutant of the present invention provides higher binding affinity than wild-type ACE2 protein, provides significantly increased coronavirus neutralization such as entry and proliferation inhibition in cells of the coronavirus, provides a wide range of the neutralization in a variant of the coronavirus, is useful for the proliferation inhibition of the coronavirus, infection prevention, and coronavirus treatment, and is particularly and usefully used as a preventive and therapeutic agent for pandemic COVID-19. 본 발명은 높은 코로나바이러스 결합 친화도 및 중화능을 가지는 안지오텐신 변환 효소 II(ACE2) 변이체, 이를 포함하는 융합단백질 및 이의 코로나바이러스의 예방 또는 치료용도에 관한 것으로, 본 발명의 안지오텐신 변환 효소 II(ACE2) 변이체는 야생형 ACE2 단백질보다 높은 결합 친화도를 나타낼뿐만 아니라, 코로나바이러스의 세포 내 진입 억제 및 증식 억제와 같은 코로나바이러스 중화능이 현저히 높으며, 코로나바이러스의 변이형에도 광범위한 중화능을 나타내어, 코로나바이러스 감염증의 증식억제, 감염 예방 및 감염증의 치료에 유용하며, 특히, 대유행 중인 COVID-19의 예방 및 치료제로 유용하게 사용될 수 있다.