고강도 감미료의 제조 방법

• Main Authors: PATRON ANDREW P, COLQUITT JUSTIN, MANAM RAMA RAO, FABER NATHAN, NORIEGA CHRIS EDANO, STEGE JUSTIN, ZIELER HELGE
• Format: Patent
• Language: Korean
• Published: 19.07.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; FOODS OR FOODSTUFFS; FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J; HUMAN NECESSITIES; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES; VINEGAR; WINE

본원에 제공된 것은 모그로사이드 화합물, 예를 들어, 화합물 1의 제조 방법, 모그로사이드 화합물을 제조하기 위한 조성물(예를 들어, 숙주 세포), 및 본원에 개시된 방법에 의해 제조된 모그로사이드 화합물, 그리고 조성물(예를 들어, 세포 용리물), 및 모그로사이드 화합물(예를 들어, 화합물 1)을 포함하는 재조합 세포를 포함한다. 또한 신규 쿠쿠르비타디에놀 신타아제에 및 이의 사용이 본원에 제공된다. Provided herein include methods of making mogroside compounds, e.g., Compound 1, compositions (for example host cells) for making the mogroside compounds, and the mogroside compounds made by the methods disclosed herein, and compositions (for example, cell lysates) and recombinant cells comprising the mogroside compounds (e.g., Compound 1). Also provided herein are novel cucurbitadienol synthases and the use thereof.