Compositon for inhibiting gene expression using CRISPRi

• Main Authors: HAN MIN WOO, SUN YOUNG CHOI
• Format: Patent
• Language: English; Korean
• Published: 29.06.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The present invention relates to a CRISPR-dCas12a vector system capable of inhibiting gene expression using CRISPR interference. By targeting cyanobacteria, the present invention can be used to control the metabolic flow of substances useful for high-efficiency photoconversion of carbon dioxide to reduce and remove carbon dioxide in an environmentally friendly manner. Compared to the conventional gene expression system, there is less time constraint and the present invention has excellent economic feasibility. Since toxicity is reduced in cyanobacteria, the present invention has advantageous properties for productivity, and has the effect of being able to be used for metabolic engineering research. 본 발명은 크리스퍼 간섭을 이용하여 유전자의 발현을 억제할 수 있는 크리스퍼-dCas12a 벡터 시스템에 관한 것으로, 시아노박테리아를 타겟으로함으로써 친환경적으로 이산화탄소를 저감 및 제거하기 위한 이산화탄소 고효율 광전화에 유용한 물질의 대사 흐름을 조절하는데 이용될 수 있으며, 종래의 유전자 발현시스템에 비해 시간 제약이 적고 경제성이 우수하며, 시아노박테리아에서 독성이 감소되므로 생산성에 유리한 특성이 있으므로, 이를 대사공학 연구에도 활용할 수 있는 효과가 있다.