식물 형질전환을 위한 방법

• Main Authors: GORDON KAMM WILLIAM JAMES, MCBRIDE KEVIN E, LOWE KEITH S, HOERSTER GEORGE J, ANAND AJITH
• Format: Patent
• Language: Korean
• Published: 11.11.2020
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

본원에 개시된 방법은 형질전환 사건의 단일 카피를 함유하는 형질전환 식물을 제공하기 위해 형태형성 유전자 또는 리프레서 유전자를 사용하고, 여기서 형태형성 유전자 또는 리프레서 유전자는 식물의 게놈으로 통합하지 않고, 식물은 벡터(플라스미드) 골격을 함유하지 않는다. Methods and compositions are provided for the improvement of double-strand-break-inducing agent activity in eukaryotic cells, through the usage of one or more morphogenic factors or developmental genes. The morphogenic factor may be provided to the same cell or to a different cell than that comprising or receiving the double-strand-break-inducing agent. The morphogenic factor may be provided to a cell as a polynucleotide composition on a recombinant vector, and may be placed on the vector outside of a T-DNA border. The morphogenic factor may be provided via an upregulation of an endogenous gene. The morphogenic factor, or the double-strand-break-inducing agent, may further comprise a cell penetrating peptide. The morphogenic factor may be co-introduced with a vector comprising RepA.