심리스 핵산 어셈블리를 위한 방법

• Main Authors: CHEN SIYUAN, LEE ELIAN, RAYNARD NATHAN, NUGENT REBECCA
• Format: Patent
• Language: Korean
• Published: 14.02.2020
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

본 출원에서 심리스 핵산 어셈블리(seamless nucleic acid assembly)를 위한 방법, 시스템, 및 조성물이 제공된다. 본 출원에서 제공된 바와 같은 방법, 시스템, 및 조성물은 프라이머 제거 없는 핵산의 효율적인 어셈블리를 제공한다. 심리스 핵산 어셈블리를 위한 방법, 시스템, 및 조성물은 경우에 따라 핵산 또는 폴리뉴클레오티드를 어셈블링하기 위한 추가의 효소와 함께 엔도뉴클레아제 또는 엑소뉴클레아제를 사용하는 것을 포함한다. Provided herein are methods, systems, and compositions for seamless nucleic acid assembly. Methods, systems, and compositions as provided herein provide for efficient assembly of nucleic acids without primer removal. Methods, systems, and compositions for seamless nucleic acid assembly comprise use of an endonuclease or exonuclease, optionally in conjunction with additional enzymes to assemble nucleic acids or polynucleotides.