분자의 다중 검출 방법

• Main Authors: HOON SHAWN, SCOLNICK JONATHAN, YEO EUGENE
• Format: Patent
• Language: Korean
• Published: 03.12.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TESTING; VINEGAR; WINE

본 발명은 일반적으로 다양한 실시양태에서 단일 샘플 또는 단일 세포에서 핵산 및 단백질과 같은 비-핵산 분자의 발현을 검출 및/또는 정량하기 위한 방법, 결합 제제 및 키트를 제공한다. 본 발명의 방법, 결합 제제 및 키트는 또한 주어진 비-핵산 분자(예를 들어, 단백질)을 발현하는 샘플에서 세포의 수를 검출 및/또는 정량하는데 유용하다. The present invention provides methods, antibodies and kits for detecting and/or quantifying expression of both non-nucleic acid molecules, such as proteins, and nucleic acid molecules in a single sample or single cell. The antibody is covalently conjugated to an oligonucleotide, such as a single-stranded DNA molecule, which comprises an identification tag and a blocking group, such as a ddNTP or an inverted dTTP, which prevents extension of the oligonucleotide by a polymerase. The method comprises the steps of reverse transcribing the conjugated oligonucleotide and the target nucleic acid simultaneously, amplifying both transcription production, and detecting the amplicons thereof. The method is also useful for detecting and/or quantifying the number of cells in a sample expressing a given non -nucleic acid molecule (e.g. protein).