레바우디오시드 E로부터 스테비올 글리코시드 레바우디오시드 D4의 생합성 제조

• Main Authors: BATTEN MICHAEL, YU XIAODAN, MAO GUOHONG, VICK JACOB EDWARD, LUO YANG, WU YILIN
• Format: Patent
• Language: Korean
• Published: 06.11.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; FOODS OR FOODSTUFFS; FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J; HUMAN NECESSITIES; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES; VINEGAR; WINE

본 발명은 일부 양태에서, 레바우디오시드 E의 사용을 통한 스테비올 글리코시드 레바우디오시드 D4의 제조에 관한 것이다. 일부 양태에서, 본 발명은 돌연변이체 CP1 효소, 돌연변이체 HV1 효소뿐만 아니라, 예컨대 레바우디오시드 D4를 제조하기 위해, 이러한 효소를 이용하는 숙주 세포 및 방법에 관한 것이다. The present invention relates, in some aspects, to the production of steviol glycoside rebaudioside D4 through the use of rebaudioside E. In some aspects, the invention relates to mutant CP1 enzymes, mutant HV1 enzymes as well as host cells and methods utilizing such enzymes, such as to produce rebaudioside D4.