H Method for Detecting Ribonuclease H Activity Using Catalytic Hairpin Assembly

• Main Authors: LEE CHANG YEOL, JUNG YUJIN, PARK HYUN GYU
• Format: Patent
• Language: English; Korean
• Published: 09.08.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The present invention relates to a method for detecting ribonuclease H (RNase H) activity using catalytic hairpin assembly. More specifically, the present invention relates to a method for detecting RNase H activity without a label with high sensitivity through catalytic hairpin assembly based signal amplification. The RNase H activity detection method based on unlabeled catalytic hairpin assembly according to the present invention does not require fluorescence and quencher material labeling, and no signal mistake occurs due to the low stability of the detection system. Therefore, it is possible to detect RNase H activity with low cost, in a simple and stable way and with high sensitivity, and to select RNase H inhibitors with potential as a treatment for AIDS. 본 발명은 헤어핀 자기조립을 이용하여 리보뉴클레아제 H (RNase H) 활성을 검출하는 방법에 관한 것으로, 더욱 자세하게는 헤어핀 자기조립 기반의 신호증폭을 통해 RNase H 활성을 표지 없이 고감도로 검출하는 방법에 관한 것이다. 본 발명에 따른 비표지 헤어핀 자기조립 기반의 RNase H 활성 검출 방법은 형광 및 소광제 물질 표지를 필요로 하지 않고, 검출 시스템의 낮은 안정성으로 인한 착오 신호 문제가 발생하지 않기 때문에, 보다 저렴하고 간편하게 안정적 및 고감도로 RNase H 활성 검출이 가능하며, 에이즈 (AIDS) 치료제로서 잠재성을 갖는 RNase H 활성저해제의 선별도 가능하다.