PEPTIDE FOR INDUCING REGENERATION OF TISSUE AND USE THEREOF

• Main Authors: TAMAI KATSUTO, NAITO KANA, YAMAZAKI TAKEHIKO, KANEZAKI TSUTOMU, NOGUCHI YUKIKO, SAKURAI SHIGERU, ENDO MAYUMI, HAMABUCHI NATSUMI
• Format: Patent
• Language: English; Korean
• Published: 26.06.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

(과제) 골수 중간엽 줄기세포 등의 PDGFRα양성 세포의 자극에 동반하여 혈중 동원, 손상 조직으로의 집적을 촉진하고 체내(in vivo)에서의 조직재생을 유도하는 치료약의 제공을 과제로 한다. (해결수단) 복수의 펩티드를 합성하고 각 펩티드의 이동 활성의 평가를 시도하였다. 그 결과 PDGFRα양성 골수 중간엽 줄기세포(MSC-1)에 대해 이동 활성을 나타내는 복수의 펩티드의 동정에 성공했다. 나아가 동정한 펩티드가 PDGFRα양성 세포인 피부 섬유아세포에 대해서도 이동 활성을 갖는 것 등을 확인하였다. The present invention concerns therapeutic agents that, in association with stimulation of PDGFR±-positive cells such as bone marrow mesenchymal stem cells, promote their mobilization into blood and accumulation in a damaged tissue, and induce tissue regeneration in a living body. In particular, fragments of High mobility Group Box 1 (HMGB1 or amphoterin) which stimulate PDGFRalpha-positive cells and promote their mobilization into blood and accumulation in a damaged tissue. Peptide fragments of HMGB1 were identified with migration-promoting activity on PDGFR-alpha-positive bone marrow mesenchymal stem cell line (MSC-1) and skin fibroblasts.