Extracting method of nucleic acid from biological samples

• Main Authors: MIN JUN HONG, BAEK CHANG YOON
• Format: Patent
• Language: English; Korean
• Published: 10.06.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The present invention relates to a method for extracting nucleic acids from a biological sample, wherein the extraction method presents a novel method for effectively extracting nucleic acids. When nucleic acids are extracted from conventional biological samples, a purification rate is low since various impurities present in the biological samples have not been properly removed. However, according to the present invention, provided is the method for extracting nucleic acids from a biological sample, of which the recovery rates of bacteria and nucleic acids are enhanced, by adding a surfactant and a sodium sulfate (Na_2SO_4) solution in a biological sample disruption step and a purification step, thereby enabling pathogens to be detected with greater sensitivity and accuracy. 본 발명은 생물학적 시료로부터 핵산을 추출하는 방법에 관한 것으로서, 상기 추출 방법은 효율적으로 핵산을 추출하기 위한 새로운 방법을 제시한다. 종래 생물학적 시료로부터 핵산을 추출할 경우, 생물학적 시료에 존재하는 다양한 불순물을 제대로 제거하지 못하여 정제율이 낮았으나, 본 발명에 의하면 생물학적 시료를 파쇄하는 단계 및 정제하는 단계에서 계면활성제 및 황산나트륨(NaSO) 용액을 첨가함으로써, 박테리아 회수율 및 핵산 회수율이 향상된 생물학적 시료로부터의 핵산 추출 방법을 제공하여, 보다 민감하고 정확하게 병원균을 검출할 수 있도록 한다.