바코딩된 올리고뉴클레오티드 서열을 갖는 시약을 이용한 단백질 발현의 측정

• Main Authors: ZOELLNER OLAF, ROHRER JURG, GAYLORD BRENT, BANSAL NIDHANJALI, LAM GRETCHEN YINBON, VIDAL JASON G, JENSEN ERIC, CHANG CHRISTINA, SHUM ELEEN, MARTIN JODY, FAN CHRISTINA, GHADIALI JAMES
• Format: Patent
• Language: Korean
• Published: 04.06.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; HEALTHCARE INFORMATICS, i.e. INFORMATION AND COMMUNICATIONTECHNOLOGY [ICT] SPECIALLY ADAPTED FOR THE HANDLING ORPROCESSING OF MEDICAL OR HEALTHCARE DATA; INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTEDFOR SPECIFIC APPLICATION FIELDS; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

본 명세서에 개시된 일부 구현예는 올리고뉴클레오티드와 컨쥬게이트된 단백질 결합 시약을 각각 포함하는 복수의 조성물들을 제공한다. 올리고뉴클레오티드는 그에 컨쥬게이트된 단백질 결합 시약에 대한 고유 식별자를 포함하고, 단백질 결합 시약은 단백질 표적에 특이적으로 결합할 수 있다. 또한, 샘플에서 복수의 단백질 표적들의 정량 분석 및 샘플에서 단백질 및 핵산 표적의 동시 정량 분석을 위한 방법 및 키트가 개시된다. 또한, 본 명세서에는 올리고뉴클레오티드와 컨쥬게이트된 단백질 결합 시약을 포함하는 표지된 생체분자제를 포함하는, 표지된 생체분자 시약을 제조하기 위한 시스템 및 방법이 개시된다. Some embodiments disclosed herein provide a plurality of compositions each comprising a protein binding reagent conjugated with an oligonucleotide. The oligonucleotide comprises a unique identifier for the protein binding reagent it is conjugated with, and the protein binding reagent is capable of specifically binding to a protein target. Further disclosed are methods and kits for quantitative analysis of a plurality of protein targets in a sample and for simultaneous quantitative analysis of protein and nucleic acid targets in a sample. Also disclosed herein are systems and methods for preparing a labeled biomolecule reagent, including a labeled biomolecule agent comprising a protein binding reagent conjugated with an oligonucleotide.