신규한 곤충 저해 단백질

• Main Authors: KESANAPALLI UMA, BOWEN DAVID J, CHAY CATHERINE A, HOWE ARLENE R
• Format: Patent
• Language: Korean
• Published: 31.05.2019
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PEST REPELLANTS OR ATTRACTANTS; PHYSICS; PLANT GROWTH REGULATORS; PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; TRAPPING; VINEGAR; WINE

나비목해충 종에 대해 독성 활성을 나타내는 살충 단백질이 개시되어 있되, 상기 살충 단백질은 TIC4472, TIC4472PL, TIC1425, TIC2613 및 TIC2613PL을 포함하지만, 이들로 제한되지는 않는다. 개시된 살충 단백질 중 하나 이상을 코딩하는 재조합 핵산 서열을 함유하는 DNA 작제물이 제공된다. 본 발명의 살충 단백질을 코딩하는 재조합 핵산 서열을 함유하는, 나비목 침입에 내성인 형질전환 식물, 식물 세포, 종자 및 식물 부분이 제공된다. 생물학적 샘플에서 본 발명의 재조합 핵산 서열 또는 단백질의 존재를 검출하는 방법 및 TIC4472, TIC4472PL, TIC1425, TIC2613 및 TIC2613PL 살충 단백질 중 어느 하나를 사용하여 나비목 종 해충을 방제하는 방법이 또한 제공된다. Pesticidal proteins exhibiting toxic activity against Lepidopteran pest species are disclosed, and include, but are not limited to, TIC4472, TIC4472PL, TIC1425, TIC2613, and TIC2613PL. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding one or more of the disclosed pesticidal proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Lepidopteran infestation are provided which contain recombinant nucleic acid sequences encoding the pesticidal proteins of the present invention. Methods for detecting the presence of the recombinant nucleic acid sequences or the proteins of the present invention in a biological sample, and methods of controlling Lepidopteran species pests using any of the TIC4472, TIC4472PL, TIC1425, TIC2613, and TIC2613PL pesticidal proteins are also provided.