Recombinant Production of Steviol Glycosides

• Main Authors: MOTION MICHAEL, HANSEN ESBEN HALKJAER, KISHORE GANESH M, HANSEN JORGEN, TAVARES SABINA, BLOM CHARLOTTE, MIKKELSEN MICHAEL DALGAARD, HICKS PAULA M, HOUGHTON LARSEN JENS
• Format: Patent
• Language: English; Korean
• Published: 26.04.2019
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; DERIVATIVES THEREOF; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; FISHING; FOODS OR FOODSTUFFS; FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J; FORESTRY; HUMAN NECESSITIES; HUNTING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC CHEMISTRY; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; SUGARS; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES; TRAPPING; VINEGAR; WINE

스테비올 생합성 효소들과 UDP-글리코실전이효소 (UGTs)을 인코드하는 신규한 재조합 유전자를 발현시키도록 조작된 재조합 미생물, 식물들, 그리고 식물 세포들을 설명한다. 이러한 미생물, 식물들, 또는 식물 세포들은 스테비올 또는 스테비올 글리코시드, 가령, 루부소시드 또는 리바우디오시드 A를 생산할 수 있고, 이는 식품 산물들 및 식이 보충제에서 천연 감미료들로 이용할 수 있다. Recombinant microorganisms, plants, and plant cells are disclosed that have been engineered to express novel recombinant genes encoding steviol biosynthetic enzymes and UDP-glycosyltransferases (UGTs). Such microorganisms, plants, or plant cells can produce steviol or steviol glycosides, e.g., rubusoside or Rebaudioside A, which can be used as natural sweeteners in food products and dietary supplements.