분자 전자 센서용 개질된 뉴클레오티드 트리포스페이트

• Main Authors: RIDEOUT DARRYL, MERRIMAN BARRY L, GEISER TIM, MOLA PAUL W
• Format: Patent
• Language: Korean
• Published: 01.04.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; DERIVATIVES THEREOF; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC CHEMISTRY; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; SUGARS; TESTING; VINEGAR; WINE

각종 구현예에서, 단일 분자 센서를 위한 신호 향상 방법이 신규한 개질된 dNTP 와 함께 개시된다. 일반적으로, 향상 방법은 폴리머라아제를 포함하는 분자 전자 센서를 제공하는 것과, 폴리머라아제에 대한 적절한 완충제 중 개질된 dNTP 및 뉴클레오티드 주형을 제공하는 것을 포함하고, 폴리머라아제 효소 작용 중 개질된 dNTP 의 혼입은, 상응하는 자연 dNTP 에 의한 성능에 비해 혼입 이벤트에 대해 향상된 전기적 신호 또는 개선된 신호-대-노이즈 비를 제공한다. In various embodiments, a signal enhancement process for single molecule molecular sensors is disclosed along with new modified dNTPs. In general, the enhancement process comprises providing a molecular electronic sensor comprising a polymerase and providing a nucleotide template and modified dNTPs in appropriate buffers to the polymerase, wherein incorporation of the modified dNTPs during action of the polymerase enzyme provides an enhanced electrical signal or improved signal-to-noise ratio for the incorporation event relative to the performance with corresponding native dNTPs.