촬영 화상 평가 장치 및 방법과, 프로그램

• Main Author: MATSUBARA KENTA
• Format: Patent
• Language: Korean
• Published: 26.03.2019
• Subjects: ACCESSORIES THEREFOR; APPARATUS OR ARRANGEMENTS EMPLOYING ANALOGOUS TECHNIQUES USINGWAVES OTHER THAN OPTICAL WAVES; APPARATUS OR ARRANGEMENTS FOR TAKING PHOTOGRAPHS OR FORPROJECTING OR VIEWING THEM; CINEMATOGRAPHY; ELECTRIC COMMUNICATION TECHNIQUE; ELECTRICITY; ELECTROGRAPHY; HOLOGRAPHY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING ANGLES; MEASURING AREAS; MEASURING IRREGULARITIES OF SURFACES OR CONTOURS; MEASURING LENGTH, THICKNESS OR SIMILAR LINEARDIMENSIONS; OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS; OPTICS; PHOTOGRAPHY; PHYSICS; PICTORIAL COMMUNICATION, e.g. TELEVISION; TESTING

적층된 배양 세포의 두께 및 밀도를, 짧은 촬영 시간으로 평가할 수 있는 촬영 화상 평가 장치 및 방법과, 프로그램을 제공한다. 대물 렌즈의 개구수가 다른 조건으로 피사체를 촬영한 촬영 화상을 취득하는 화상 취득부(52)와, 상대적으로 대물 렌즈의 개구수가 작은 조건으로 촬영된 저NA 촬영 화상에 근거하여, 피사체의 두께를 추정하는 두께 추정부(53)과, 상대적으로 대물 렌즈의 개구수가 큰 조건으로 촬영된 고NA 촬영 화상에 근거하여, 피사체의 밀도를 추정하는 밀도 추정부(54)를 구비한다. Provided are a captured image evaluation apparatus, a captured image evaluation method, and a captured image evaluation program capable of evaluating a thickness and a density of stacked cultured cells in a short imaging time. The captured image evaluation apparatus includes: an image acquisition section 52 that acquires captured images obtained by imaging a subject under a condition in which a numerical aperture of an objective lens is changed; a thickness estimation section 53 that estimates a thickness of the subject on the basis of a low NA captured image obtained under a condition in which the numerical aperture of the objective lens is relatively small; and a density estimation section 54 that estimates a density of the subject on the basis of a high NA captured image obtained under a condition in which the numerical aperture of the objective lens is relatively large.