대류 유체 디바이스에서의 핵산의 표면-기반 검출

• Main Authors: KHODAKOV DMITRIY A, ZHANG DAVID
• Format: Patent
• Language: Korean
• Published: 05.03.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PERFORMING OPERATIONS; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TRANSPORTING; VINEGAR; WINE

본 발명은 핵산 서열의 대류-기반 PCR 및 비효소적 증폭을 수행하기 위한 방법, 조성물 및 디바이스를 제공한다. 이들 방법에 사용되는 기법 및 시약은 핵 시험 및 검정에 사용하기 위한, 개방 및 폐쇄 시스템에서의, 토홀드 프로브(toehold probe), 가닥 치환 반응, 레일리-베나드 대류(Rayleigh-Benard convection), 온도 구배, 멀티플렉스 증폭, 멀티플렉스 검출, 및 DNA 기능화를 포함한다. The present disclosure provides methods, composition and devices for performing convection-based PCR and non-enzymatic amplification of nucleic acid sequences. Techniques and reagents employed in these methods include toehold probes, strand displacement reactions, Rayleigh-Benard convection, temperature gradients, multiplexed amplification, multiplexed detection, and DNA functionalization, in open and closed systems, for use in nucleic tests and assays.