Analytic method of fatty acids with improved sensitivity and specificity

• Main Authors: LEE, KYOUNG RYUL, KIM, SEOL A, LEE, SANG HOO, KIM, JU HOON, PARK, JI MYEONG
• Format: Patent
• Language: English; Korean
• Published: 12.09.2018
• Subjects: BASIC ELECTRIC ELEMENTS; ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS; ELECTRICITY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The present invention relates to a fatty acid quantifying method, and more specifically, to an improved method for simultaneously quantifying fatty acids in a driven blood spot (DBS) with LC/ESI-MS/MS by picolylamine derivatization. An analysis method according to the present invention is advantageous in that storage and sample stability are superior than the conventional blood specimen by using the DBS as a specimen to be analyzed. Also, the analysis method of the present invention is advantageous as compared with the conventional analysis method using blood in that using the DBS as a specimen causes less stress than blood collection and enables easy sample collection by an ordinary person, thereby being used as a point-of-care test kit. In addition, it is possible to simultaneously quantify fatty acids in DBS with LC/ESI-MS/MS by picolylamine derivatization, and to have not only higher sensitivity and specificity but also reproducibility and repeatability than the conventional non-derivatization method. 본 발명은 지방산 정량 방법에 관한 것으로서, 보다 상세하게는 혈액여지(dried blood spot, DBS) 중의 지방산을 피콜릴아민(picolylamine) 유도체화하여 LC/ESI-MS/MS로 동시 정량할 수 있는 개선된 방법에 관한 것이며, 본 발명에 따른 분석방법은 혈액여지를 분석대상 검체로 사용함으로써, 보관 및 시료안정성이 기존 혈액 검체보다 우수하다는 장점이 있고, 혈액 채취에 비하여 스트레스가 적고 일반인에 의한 용이한 시료 채취가 가능하므로 현장검사(point-of-care test) 키트로 활용이 가능하다는 점에서 기존 혈액을 이용한 분석방법에 비하여 유리하며, 혈액여지 중의 지방산을 피콜릴아민 유도체함으로써 LC/ESI-MS/MS로 동시 정량이 가능하고, 기존의 비유도체화 방법에 비하여 고감도이면서 특이성이 높을 뿐만 아니라 재현성 및 반복성도 우수하다는 유리한 효과를 갖는다.