A mutant of Neosartorya fischeri betaglucosidase improved in its activity and use of the same

• Main Authors: LEE, JUNG KUL, LI JINGLIN, JEONG, SEONG HUN
• Format: Patent
• Language: English; Korean
• Published: 08.03.2018
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMES; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The present invention relates to a mutant beta-glucosidase derived from Neosartorya fischeri with enhanced activities, and a use thereof. According to the present invention, the mutant beta-glucosidase derived from Neosartorya fischeri with improved activities ensures improved enzymatic efficiency since a residue playing a crucial role in the activities of beta-glucosidase derived from Neosartorya fischeri is mutated. Accordingly, it is possible to provide a method to efficiently saccharify substrates when the mutant beta-glucosidase derived from Neosartorya fischeri with improved activities is used. 본 발명은 활성이 개선된 네오사토리아 피쉐리 유래의 돌연변이 베타-글루코시다제 및 그 용도에 관한 것이다. 본 발명에 따른 활성이 개선된 네오사토리아 피쉐리 유래의 돌연변이 베타-글루코시다제는 네오사토리아 피쉐리 유래의 베타-글루코시다제의 활성에 있어서 중요한 역할을 하는 잔기가 돌연변이 되어 효소의 효율이 증가되며, 상기 활성이 개선된 네오사토리아 피쉐리 유래의 돌연변이 베타-글루코시다제를 이용할 경우 효율적으로 기질을 당화하는 방법을 제공할 수 있다.